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Experiment

Id
 
Name
Adhesion of water stressed Helicobacter pylori to abiotic surfaces  
Is Interexperiment
compoundExperimentNo  
Created
05/06/2020  
Published
Contributors
Nuno Azevedo  
State
Experiment accepted

Description

The main aim of this work was to study and compare the adhesion of water exposed Helicobacter pylori to six different substrata and correlate any changes in morphology, physiology, ability to form aggregates and cultivability when in the planktonic or in the sessile phase. The fact that the pathogen is able to adhere, retain the spiral morphology for longer and form large aggregates when attached to different plumbing materials appeared to point to pipe materials in general, and copper plumbing in particular, as a possible reservoir of virulent H. pylori in water distribution systems. However, the Syto9/PI staining results and cultivability methods indicate that the attached H. pylori cells quickly enter in a nonviable physiological state. This represents the first study of H. pylori behaviour in water-exposed abiotic surfaces. It suggests that co-aggregation with the autochthonous heterotrophic consortia present in water is necessary for a longer survival of the pathogen in biofilms associated to drinking water systems.

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Conditions

Name Value Units
shear force 0 rpm
medium water NULL
organism Helicobacter pylori NULL
device 6-well microtiter plate with coupons NULL
temperature 24 ºc

Methods

4',6-diamidino-2-phenylindole staining - -

Name Value Units
material copper, glass, Polipropilene, polyvinyl chloride, stainless steel grade SS304, stainless steel grade SS316 NULL
time 2, 6, 13, 24, 48, 96, 192 h
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