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Experiment

Id
 
Name
Adhesion Influence of Water-Stressed Helicobacter pylori to SS304 and polypropylene  
Is Interexperiment
compoundExperimentNo  
Created
31/03/2020  
Published
Contributors
Nuno Azevedo  
State
Experiment accepted

Description

This study aimed to investigate the influence of physiological states of Pseudomonas aeruginosa and Escherichia coli in cell-to-cell interactions. Conditioned media obtained either in biofilm or in planktonic mode of life triggered a synergistic effect on planktonic growth, mainly for E. coli single cultures growing in P. aeruginosa supernatants. Biofilms grown in the presence of P. aeruginosa biofilms-derived metabolites presented less mass and activity. These events highlight that, when developed in biofilm, P. aeruginosa release signals or metabolites able to prejudice single and binary biofilm growth of others species and of their own species. However, products released by their planktonic counterparts did not impair biofilm growth or activity. E. coli, living as planktonic or sessile cultures, released signals and metabolites or removed un-beneficial compounds which promoted the growth and activity of all the species. Our findings revealed that inter and intraspecies behaviors depend on the involved bacteria and their adopted mode of life.

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Conditions

Name Value Units
medium water NULL
organism Helicobacter pylori NULL
device 6-well microtiter plate with coupons NULL

Methods

colony-forming unit - -

Name Value Units
antimicrobial agent rifampicin NULL
shear force 0, 60, 120 rpm
material polypropylene, stainless steel grade SS304 NULL
time 2, 6, 14, 24, 48, 96, 192 h
temperature 4, 23, 37 ºc
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